CD4, also known as T-cell surface antigen T4, Leu-3, L3T4, and W3/25, is a single-pass type I transmembrane glycoprotein of the immunoglobulin superfamily (IgSF). CD4 contains 3 Ig- like C2-type (IgC2) domains and 1 Ig-like V-type (IgV) domain in its extracellular region. CD4 is expressed predominantly on thymocytes and a subset of mature T lymphocytes.  CD4 serves as a standard marker for the identification of T cell populations.  CD4 is expressed along with CD8 on T cells during their early development in the thymus. CD4 or CD8 expression is then lost, giving rise to single positive CD4+ or CD8+ mature T cells. CD4+ cells, also known as T helper (Th) cells, further differentiate into multiple subsets including Th1, Th2, Th17, Tfh, and Treg cells, which are crucial in the regulation of humoral and cellular immunity. In human, CD4 is also expressed on macrophages, neutrophils, monocytes, NK cells, dendritic cells and glial cells in the brain.  CD4 is a co-receptor that assists the T cell receptor (TCR) to activate T cells following the interaction with MHC class II molecules on antigen presenting cells (APC). CD4 also functions as a chemotactic receptor for IL­16. In human, CD4 also acts as a co-receptor for the gp120 surface glycoprotein of HIV­1. CD4 is a primary receptor by HIV-1 to gain entry into host T cells. 

CD4, also known as T-cell surface antigen T4, Leu-3, L3T4, and W3/25, is a single-pass type I transmembrane glycoprotein of the immunoglobulin superfamily (IgSF). CD4 contains 3 Ig- like C2-type (IgC2) domains and 1 Ig-like V-type (IgV) domain in its extracellular region. CD4 is expressed predominantly on thymocytes and a subset of mature T lymphocytes.  CD4 serves as a standard marker for the identification of T cell populations.  CD4 is expressed along with CD8 on T cells during their early development in the thymus. CD4 or CD8 expression is then lost, giving rise to single positive CD4+ or CD8+ mature T cells. CD4+ cells, also known as T helper (Th) cells, further differentiate into multiple subsets including Th1, Th2, Th17, Tfh, and Treg cells, which are crucial in the regulation of humoral and cellular immunity. In human, CD4 is also expressed on macrophages, neutrophils, monocytes, NK cells, dendritic cells and glial cells in the brain.  CD4 is a co-receptor that assists the T cell receptor (TCR) to activate T cells following the interaction with MHC class II molecules on antigen presenting cells (APC). CD4 also functions as a chemotactic receptor for IL­16. In human, CD4 also acts as a co-receptor for the gp120 surface glycoprotein of HIV­1. CD4 is a primary receptor by HIV-1 to gain entry into host T cells. 

 

Product Details

 

Gene Symbol: CD4; T4/Leu-3; CD4mut;

 

NCBI Gene ID: 920

 

Uniprot Entry: P01730

 

Construct Details: Full length human CD4 gene (encoding UniProt #P01730) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1377 (bp)

 

Gene Insert Sequence:  

ATGAACCGGGGAGTCCCTTTTAGGCACTTGCTTCTGGTGCTGCAACTGGCGCTCCTCCCAGCAGCCACTCAGGGAAAGAA

AGTGGTGCTGGGCAAAAAAGGGGATACAGTGGAACTGACCTGTACAGCTTCCCAGAAGAAGAGCATACAATTCCACTGGA

AAAACTCCAACCAGATAAAGATTCTGGGAAATCAGGGCTCCTTCTTAACTAAAGGTCCATCCAAGCTGAATGATCGCGCT

GACTCAAGAAGAAGCCTTTGGGACCAAGGAAACTTTCCCCTGATCATCAAGAATCTTAAGATAGAAGACTCAGATACTTA

CATCTGTGAAGTGGAGGACCAGAAGGAGGAGGTGCAATTGCTAGTGTTCGGATTGACTGCCAACTCTGACACCCACCTGC

TTCAGGGGCAGAGCCTGACCCTGACCTTGGAGAGCCCCCCTGGTAGTAGCCCCTCAGTGCAATGTAGGAGTCCAAGGGGT

AAAAACATACAGGGGGGGAAGACCCTCTCCGTGTCTCAGCTGGAGCTCCAGGATAGTGGCACCTGGACATGCACTGTCTT

GCAGAACCAGAAGAAGGTGGAGTTCAAAATAGACATCGTGGTGCTAGCTTTCCAGAAGGCCTCCAGCATAGTCTATAAGA

AAGAGGGGGAACAGGTGGAGTTCTCCTTCCCACTCGCCTTTACAGTTGAAAAGCTGACGGGCAGTGGCGAGCTGTGGTGG

CAGGCGGAGAGGGCTTCCTCCTCCAAGTCTTGGATCACCTTTGACCTGAAGAACAAGGAAGTGTCTGTAAAACGGGTTAC

CCAGGACCCTAAGCTCCAGATGGGCAAGAAGCTCCCGCTCCACCTCACCCTGCCCCAGGCCTTGCCTCAGTATGCTGGCT

CTGGAAACCTCACCCTGGCCCTTGAAGCGAAAACAGGAAAGTTGCATCAGGAAGTGAACCTGGTGGTGATGAGAGCCACT

CAGCTCCAGAAAAATTTGACCTGTGAGGTGTGGGGACCCACCTCCCCTAAGCTGATGCTGAGTTTGAAACTGGAGAACAA

GGAGGCAAAGGTCTCGAAGCGGGAGAAGGCGGTGTGGGTGCTGAACCCTGAGGCGGGGATGTGGCAGTGTCTGCTGAGTG

ACTCGGGACAGGTCCTGCTGGAATCCAACATCAAGGTTCTGCCCACATGGTCCACCCCGGTGCAGCCAATGGCCCTGATT

GTGCTGGGGGGCGTCGCCGGCCTCCTGCTTTTCATTGGGCTAGGCATCTTCTTCTGTGTCAGGTGCCGGCACCGAAGGCG

CCAAGCAGAGCGGATGTCTCAGATCAAGAGACTCCTCAGTGAGAAGAAGACCTGCCAGTGTCCTCACCGGTTTCAGAAGA

CATGTAGCCCCATTTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Nature 312:763 & 767 (1984)

2. Cell 42:93 (1985)

3. Proc. Natl. Acad. Sci. 83: 4399 (1986)

4. Nature 330:256 (1987)

5. Structure 2 : 59 (1994)

6. Nature 463:963 (2010)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP2275-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.