AGRB1 or BAI1 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. BAI1 is an orphan receptor involved in cell adhesion and probably in cell-cell interactions.  It is a brain-specific angiogenesis inhibitor (also known as BAI1). BAI1 contains at least one 'functional' p53-binding site within an intron, and its expression has been shown to be induced by wildtype p53. There are two other brain-specific angiogenesis inhibitor genes, designated BAI2 and BAI3 which along with BAI1 have similar tissue specificities and structures. BAI1 is postulated to be an inhibitor of angiogenesis and a growth suppressor of glioblastomas.

AGRB1 or BAI1 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. BAI1 is an orphan receptor involved in cell adhesion and probably in cell-cell interactions.  It is a brain-specific angiogenesis inhibitor (also known as BAI1). BAI1 contains at least one 'functional' p53-binding site within an intron, and its expression has been shown to be induced by wildtype p53. There are two other brain-specific angiogenesis inhibitor genes, designated BAI2 and BAI3 which along with BAI1 have similar tissue specificities and structures. BAI1 is postulated to be an inhibitor of angiogenesis and a growth suppressor of glioblastomas.

 

 

Product Details

 

Gene Symbol: BAI1; ADGRB1; GDAIF

 

NCBI Gene ID: 575

 

Uniprot Entry: O14514

 

Construct Details: Full length human BAI1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 4755 (bp)

 

Gene Insert Sequence:  

ATGAGGGGCCAGGCCGCCGCCCCGGGCCCCGTCTGGATCCTCGCCCCGCTGCTACTGCTGCTGCTGCTGC

TGGGACGCCGCGCGCGGGCGGCCGCCGGAGCAGACGCGGGGCCCGGGCCCGAGCCGTGCGCCACGCTGGT

GCAGGGAAAGTTCTTCGGCTACTTCTCCGCGGCCGCCGTGTTCCCGGCCAACGCCTCGCGCTGCTCCTGG

ACGCTACGCAACCCGGACCCGCGGCGCTACACTCTCTACATGAAGGTGGCCAAGGCGCCCGTGCCCTGCA

GCGGCCCCGGCCGCGTGCGCACCTACCAGTTCGACTCCTTCCTCGAGTCCACGCGCACCTACCTGGGCGT

GGAGAGCTTCGACGAGGTGCTGCGGCTCTGCGACCCCTCCGCACCCCTGGCCTTCCTGCAGGCCAGCAAG

CAGTTCCTGCAGATGCGGCGCCAGCAGCCGCCCCAGCACGACGGGCTCCGGCCCCGGGCCGGGCCGCCGG

GCCCCACCGACGACTTCTCCGTGGAGTACCTGGTGGTGGGGAACCGCAACCCCAGCCGTGCCGCCTGCCA

GATGCTGTGCCGCTGGCTGGACGCGTGTCTGGCCGGTAGTCGCAGCTCGCACCCCTGCGGGATCATGCAG

ACCCCCTGCGCCTGCCTGGGCGGCGAGGCGGGCGGCCCTGCCGCGGGACCCCTGGCCCCCCGCGGGGATG

TCTGCTTGAGAGATGCGGTGGCTGGTGGCCCTGAAAACTGCCTCACCAGCCTGACCCAGGACCGGGGCGG

GCACGGCGCCACAGGCGGCTGGAAGCTGTGGTCCCTGTGGGGCGAATGCACGCGGGACTGCGGGGGAGGC

CTCCAGACGCGGACGCGCACCTGCCTGCCCGCGCCGGGCGTGGAGGGCGGCGGCTGCGAGGGGGTGCTGG

AGGAGGGTCGCCAGTGCAACCGCGAGGCCTGCGGCCCCGCTGGGCGCACCAGCTCCCGGAGCCAGTCCCT

GCGGTCCACAGATGCCCGGCGGCGCGAGGAGCTGGGGGACGAGCTGCAGCAGTTTGGGTTCCCAGCCCCC

CAGACCGGTGACCCAGCAGCCGAGGAGTGGTCCCCGTGGAGCGTGTGCTCCAGCACCTGCGGCGAGGGCT

GGCAGACCCGCACGCGCTTCTGCGTGTCCTCCTCCTACAGCACGCAGTGCAGCGGACCCCTGCGCGAGCA

GCGGCTGTGCAACAACTCTGCCGTGTGCCCAGTGCATGGTGCCTGGGATGAGTGGTCGCCCTGGAGCCTC

TGCTCCAGCACCTGTGGCCGTGGCTTTCGGGATCGCACGCGCACCTGCAGGCCCCCCCAGTTTGGGGGCA

ACCCCTGTGAGGGCCCTGAGAAGCAAACCAAGTTCTGCAACATTGCCCTGTGCCCTGGCCGGGCAGTGGA

TGGAAACTGGAATGAGTGGTCGAGCTGGAGCGCCTGCTCCGCCAGCTGCTCCCAGGGCCGACAGCAGCGC

ACGCGTGAATGCAACGGGCCTTCCTACGGGGGTGCGGAGTGCCAGGGCCACTGGGTGGAGACCCGAGACT

GCTTCCTGCAGCAGTGCCCAGTGGATGGCAAGTGGCAGGCCTGGGCGTCATGGGGCAGTTGCAGCGTCAC

GTGTGGGGCTGGCAGCCAGCGACGGGAGCGTGTCTGCTCTGGGCCCTTCTTCGGGGGAGCAGCCTGCCAG

GGCCCCCAGGATGAGTACCGGCAGTGCGGCACCCAGCGGTGTCCCGAGCCCCATGAGATCTGTGATGAGG

ACAACTTTGGTGCTGTGATCTGGAAGGAGACCCCAGCGGGAGAGGTGGCTGCTGTCCGGTGTCCCCGCAA

CGCCACAGGACTCATCCTGCGACGGTGTGAGCTGGACGAGGAAGGCATCGCCTACTGGGAGCCCCCCACC

TACATCCGCTGTGTTTCCATTGACTACAGAAACATCCAGATGATGACCCGGGAGCACCTGGCCAAGGCTC

AGCGAGGGCTGCCTGGGGAGGGGGTCTCGGAGGTCATCCAGACACTGGTGGAGATCTCTCAGGACGGGAC

CAGCTACAGTGGGGACCTGCTGTCCACCATCGATGTCCTGAGGAACATGACAGAGATTTTCCGGAGAGCG

TACTACAGCCCCACCCCTGGGGACGTACAGAACTTTGTCCAGATCCTTAGCAACCTGTTGGCAGAGGAGA

ATCGGGACAAGTGGGAGGAGGCCCAGCTGGCGGGCCCCAACGCCAAGGAGCTGTTCCGGCTGGTGGAGGA

CTTTGTGGACGTCATCGGCTTCCGCATGAAGGACCTGAGGGATGCATACCAGGTGACAGACAACCTGGTT

CTCAGCATCCATAAGCTCCCAGCCAGCGGAGCCACTGACATCAGCTTCCCCATGAAGGGCTGGCGGGCCA

CGGGTGACTGGGCCAAGGTGCCAGAGGACAGGGTCACTGTGTCCAAGAGTGTCTTCTCCACGGGGCTGAC

AGAGGCCGATGAAGCATCCGTGTTTGTGGTGGGCACCGTGCTCTACAGGAACCTGGGCAGCTTCCTGGCC

CTGCAGAGGAACACGACCGTCCTGAATTCTAAGGTGATCTCCGTGACTGTGAAACCCCCGCCTCGCTCCC

TGCGCACACCCTTGGAGATCGAGTTTGCCCACATGTATAATGGCACCACCAACCAGACCTGTATCCTGTG

GGATGAGACGGATGTACCCTCCTCCTCCGCCCCCCCGCAGCTCGGGCCCTGGTCGTGGCGCGGCTGCCGC

ACGGTGCCCCTCGACGCCCTCCGGACGCGCTGCCTCTGTGACCGGCTCTCCACCTTCGCCATCTTAGCCC

AGCTCAGCGCCGACGCGAACATGGAGAAGGCGACTCTGCCGTCGGTGACGCTCATCGTGGGCTGTGGCGT

GTCCTCTCTCACCCTGCTCATGCTGGTCATCATCTACGTGTCCGTGTGGAGGTACATTCGCTCAGAGCGT

TCTGTCATCCTCATCAACTTCTGCCTGTCCATCATCTCCTCCAATGCCCTCATCCTCATCGGGCAGACCC

AGACCCGCAACAAGGTGGTGTGCACGCTGGTGGCCGCCTTCCTGCACTTCTTCTTCCTGTCCTCCTTCTG

CTGGGTGCTCACCGAGGCCTGGCAGTCCTACATGGCGGTGACGGGCCACCTCCGGAACCGCCTCATCCGC

AAGCGCTTCCTCTGCCTGGGCTGGGGGCTCCCTGCACTGGTTGTGGCCATTTCTGTGGGATTCACCAAGG

CCAAAGGGTACAGCACCATGAACTACTGCTGGCTCTCCCTGGAGGGGGGACTGCTCTATGCCTTCGTGGG

ACCTGCCGCTGCCGTTGTGCTGGTGAACATGGTCATTGGGATCCTGGTGTTCAACAAGCTCGTGTCCAAA

GACGGCATCACGGACAAGAAGCTGAAGGAGCGGGCAGGGGCCTCCCTGTGGAGCTCCTGCGTGGTGCTGC

CGCTGCTGGCGCTGACCTGGATGTCGGCTGTGCTCGCCGTCACCGACCGCCGCTCCGCCCTCTTCCAGAT

CCTCTTCGCTGTCTTCGACTCGCTGGAGGGCTTCGTCATCGTCATGGTGCACTGTATCCTCCGTAGAGAG

GTCCAGGACGCTGTGAAATGCCGTGTGGTTGACCGGCAGGAGGAGGGCAACGGGGACTCAGGGGGCTCCT

TCCAGAACGGCCACGCCCAGCTCATGACCGACTTCGAGAAGGACGTGGATCTGGCCTGTAGATCAGTGCT

GAACAAGGACATCGCGGCCTGCCGCACTGCCACCATCACGGGCACACTGAAGCGGCCGTCTCTGCCCGAG

GAGGAGAAGCTGAAGCTGGCCCATGCCAAGGGGCCGCCCACCAATTTCAACAGCCTGCCGGCCAACGTGT

CCAAGCTGCACCTGCACGGCTCACCCCGCTATCCCGGCGGGCCCCTGCCCGACTTCCCCAACCACTCACT

GACCCTCAAGAGGGACAAGGCGCCCAAGTCCTCCTTCGTCGGTGACGGGGACATCTTCAAGAAGCTGGAC

TCGGAGCTGAGCCGGGCCCAGGAGAAGGCTCTGGACACGAGCTACGTGATCCTGCCCACGGCCACGGCCA

CGCTGCGGCCCAAGCCCAAGGAGGAGCCCAAGTACAGCATCCACATTGACCAGATGCCGCAGACCCGCCT

CATCCACCTCAGCACGGCCCCCGAGGCCAGCCTCCCCGCCCGCAGCCCGCCCTCCCGCCAGCCCCCCAGC

GGCGGGCCCCCCGAGGCACCCCCTGCCCAGCCCCCACCGCCTCCGCCCCCACCGCCACCACCTCCCCAGC

AGCCCCTGCCCCCACCGCCCAATCTGGAGCCGGCACCCCCCAGCCTGGGGGATCCCGGGGAGCCTGCCGC

CCATCCGGGACCCAGCACGGGGCCCAGCACCAAGAACGAGAATGTCGCCACCTTGTCTGTGAGCTCCCTG

GAGCGGCGGAAGTCGCGGTATGCAGAACTGGACTTTGAGAAGATCATGCACACCCGGAAGCGGCACCAAG

ACATGTTCCAGGACCTGAACCGGAAGCTGCAGCACGCAGCGGAGAAGGACAAGGAGGTGCTGGGGCCGGA

CAGCAAGCCGGAAAAGCAGCAGACGCCCAACAAGAGGCCCTGGGAGAGCCTCCGGAAAGCCCACGGGACG

CCCACGTGGGTGAAGAAGGAGCTGGAGCCGCTGCAGCCGTCGCCGCTGGAGCTTCGCAGCGTGGAGTGGG

AGAGGTCGGGCGCCACGATCCCGCTGGTGGGCCAGGACATCATCGACCTCCAGACCGAGGTCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

1. Ann Rev Biochem 56: 615 (1987)

2. Genome Biol. 2: S3013 (2001)

3. Science 301: 361 (2003)

4. Physiol Rev 85: 1159 (2005)

5. Genomics 88: 263 (2006)

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.