AGRG3 is a 7 transmembrane (7TM) receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRG3 may be proteolytically processed to form a heterodimer of 2 chains: the large extracellular N-terminal fragment and the membrane-bound C-terminal 7TM fragment predominantly remain associated and non-covalently linked.  AGRG3 is expressed in cultured primary dermal lymphatic endothelial cells. AGRG3 is an orphan receptor that may regulate the migration of lymphatic endothelial cells. It may also regulate B-cell development.

AGRG3 is a 7 transmembrane (7TM) receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRG3 may be proteolytically processed to form a heterodimer of 2 chains: the large extracellular N-terminal fragment and the membrane-bound C-terminal 7TM fragment predominantly remain associated and non-covalently linked.  AGRG3 is expressed in cultured primary dermal lymphatic endothelial cells. AGRG3 is an orphan receptor that may regulate the migration of lymphatic endothelial cells. It may also regulate B-cell development.

 

Product Details

 

Gene Symbol: AGRG3; ADGRG3; GPR97; PGR26; PB99

 

NCBI Gene ID: 222487

 

Uniprot Entry: Q86Y34

 

Construct Details: Full length human AGRG3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1650 (bp)

 

Gene Insert Sequence:  

ATGGCGACGCCCAGGGGCCTGGGGGCCCTGCTCCTGCTCCTCCTGCTCCCGACCTCAGGTCAGGAAAAGC

CCACCGAAGGGCCAAGAAACACCTGCCTGGGGAGCAACAACATGTACGACATCTTCAACTTGAATGACAA

GGCTTTGTGCTTCACCAAGTGCAGGCAGTCGGGCAGCGACTCCTGCAATGTGGAAAACTTGCAGAGATAC

TGGCTAAACTACGAGGCCCATCTGATGAAGGAAGGTTTGACGCAGAAGGTGAACACGCCTTTCCTGAAGG

CTTTGGTCCAGAACCTCAGCACCAACACTGCAGAAGACTTCTATTTCTCTCTGGAGCCCTCTCAGGTTCC

GAGGCAGGTGATGAAGGACGAGGACAAGCCCCCTGACAGAGTGCGACTTCCCAAGAGCCTTTTTCGATCC

CTGCCAGGCAACAGGTCTGTGGTCCGCTTGGCCGTCACCATTCTGGACATTGGTCCAGGGACTCTCTTCA

AGGGCCCCCGGCTCGGCCTGGGAGATGGCAGCGGCGTGTTGAACAATCGCCTGGTGGGTTTGAGTGTGGG

ACAAATGCATGTCACCAAGCTGGCTGAGCCTCTGGAGATCGTCTTCTCTCACCAGCGACCGCCCCCTAAC

ATGACCCTCACCTGTGTATTCTGGGATGTGACTAAAGGGACCACTGGAGACTGGTCTTCTGAGGGCTGCT

CCACGGAGGTCAGACCTGAGGGGACCGTGTGCTGCTGTGACCACCTGACCTTTTTCGCCCTGCTCCTGAG

ACCCACCTTGGACCAGTCCACGGTGCATATCCTCACACGCATCTCCCAGGCGGGCTGTGGGGTCTCCATG

ATCTTCCTGGCCTTCACCATTATTCTTTATGCCTTTCTGAGGCTTTCCCGGGAGAGGTTCAAGTCAGAAG

ATGCCCCAAAGATCCACGTGGCCCTGGGTGGCAGCCTGTTCCTCCTGAATCTGGCCTTCTTGGTCAATGT

GGGGAGTGGCTCAAAGGGGTCTGATGCTGCCTGCTGGGCCCGGGGGGCTGTCTTCCACTACTTCCTGCTC

TGTGCCTTCACCTGGATGGGCCTTGAAGCCTTCCACCTCTACCTGCTCGCTGTCAGGGTCTTCAACACCT

ACTTCGGGCACTACTTCCTGAAGCTGAGCCTGGTGGGCTGGGGCCTGCCCGCCCTGATGGTCATCGGCAC

TGGGAGTGCCAACAGCTACGGCCTCTACACCATCCGTGATAGGGAGAACCGCACCTCTCTGGAGCTATGC

TGGTTCCGTGAAGGGACAACCATGTACGCCCTCTATATCACCGTCCACGGCTACTTCCTCATCACCTTCC

TCTTTGGCATGGTGGTCCTGGCCCTGGTGGTCTGGAAGATCTTCACCCTGTCCCGTGCTACAGCGGTCAA

GGAGCGGGGGAAGAACCGGAAGAAGGTGCTCACCCTGCTGGGCCTCTCGAGCCTGGTGGGTGTGACATGG

GGGTTGGCCATCTTCACCCCGTTGGGCCTCTCCACCGTCTACATCTTTGCACTTTTCAACTCCTTGCAAG

GTGTCTTCATCTGCTGCTGGTTCACCATCCTTTACCTCCCAAGTCAGAGCACCACAGTCTCCTCCTCTAC

TGCAAGATTGGACCAGGCCCACTCCGCATCTCAAGAATAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

1. Ann Rev Biochem 56: 615 (1987)

2. Genome Biol. 2: S3013 (2001)

3. Science 301: 361 (2003)

4. Physiol Rev 85: 1159 (2005)

5. Genomics 88: 263 (2006)

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.