Overview: Our Technology

G&P Biosciences benefits greatly from a number of technical advantages, providing the best chance of expressing a protein in soluble, active form with high yield. We have experience and expertise in all stages of protein expression, starting from codon optimization, vector/promoter choice, tag/fusion use, culture medium, additives, feedings, host cell engineering, stable cell line creation to protein purification and formulation (see a typical workflow below). We develop unique, high throughput, mammalian cell expression systems, allowing exploiting every technique to improve the productivity for recombinant protein expression. With innovative solutions for varying conditions & parameters, we can quickly deliver recombinant proteins with the highest qualities possible to our clients in a short turnaround time. 

We offers custom recombinant protein expression and purification using mammalian cell expression systems (including HEK293 and CHO) for small or large quantities (up to grams) of protein production. Our unique mammalian cell expression systems that are based on our high-throughput gene expression technologies, exhibit significantly improved success rates and production yields.  Our custom-made recombinant proteins are expressed in mammalian cells growing under animal component-free, antibiotic-free condition, purified to the highest homogeneity possible, and formulated into the carrier-free or customer-specified buffer suitable for various downstream in vitro & in vivo assays (visit our Protein Products and Protein Services pages to learn more and order).

Antibody Production Technology & Capability

G&P Biosciences has developed unique mammalian expression systems for recombinant antibody production in a high throughput and time effective manner, especially suitable for research laboratory needs. We have exploited a large panel of expression vectors and selection methods for stable antibody cell line generation. Our expression vectors are designed to allow high throughput cloning of immunoglobulin genes and subsequent expression as whole antibodies or fragments. We can express a variety of different class and subclasses of IgG (e.g., human IgG1-4 including some allotypic variants) from many species, including human, mouse and rabbit. We can also produce many Fv and Fab fragment-derived molecules, such as Fab, Fab’, F(ab)’2, “minibody”, scFv-Fc and bispecific antibodies.

We also provide a range of antibody engineering services that may complement your research and accelerate the progress of development towards clinical use. Our services are offered as stand-alone services and also as part of a complete suite of antibody custom solution package. We offer a proprietary antibody humanization service. Using our technology, the sequences of the antibody variable domains, which determine its binding specificity, are incorporated into human donor sequences properly, thus creating a panel of humanized antibodies for testing. We also provide bundled services, starting from antigen preparation, hybridoma screening, recombinant antibody generation, affinity determination, antibody humanization and affinity maturation to custom-scale production. We can drive your antibody R&D from any stage to the delivery of a 100% royalty free drug candidate (visit our Antibody Products and Antibody Services to learn more and order).

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