CD66a, also known as CEACAM1 (carcinoembryonic antigen (CEA)-related cell adhesion molecule 1) and  BGP (biliary glycoprotein), is a single pass type I tranmembrane protein of the immunoglobulin superfamily (IgSF). CEA family members are either transmembrane or GPI­anchored within two subgroups: the CEACAMs and the pregnancy-specific glycoproteins.  In human, 7 CEACAMs have been identified including 3 transmembrane proteins (CEACAM1, 3, 4) and 4 GPI-anchored molecules (CEACAM5-8). Human CEACAM1 contains 1 Ig-like V-type (IgV) motif and 3 Ig-like C2-type (IgC2) motifs in the N-terminal extracellular domain (ECD), followed by a transmembrane domain (TM) and an ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 has 3 soluble and 7 transmembrane isoforms due to alternate splicing. The functions of these isoforms are not clear. CEACAM1 is an adhesion molecule mediating cell adhesion via both homotypic and heterotypic associations with CEACAM5/CEA and CEACAM6/NCA.  CEACAM1 is highly glycosylated and can be detected on leukocytes, epithelia, and endothelia. CEACAM1 is involved in multiple cellular processes including differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.

CD66a, also known as CEACAM1 (carcinoembryonic antigen (CEA)-related cell adhesion molecule 1) and  BGP (biliary glycoprotein), is a single pass type I tranmembrane protein of the immunoglobulin superfamily (IgSF). CEA family members are either transmembrane or GPI­anchored within two subgroups: the CEACAMs and the pregnancy-specific glycoproteins.  In human, 7 CEACAMs have been identified including 3 transmembrane proteins (CEACAM1, 3, 4) and 4 GPI-anchored molecules (CEACAM5-8). Human CEACAM1 contains 1 Ig-like V-type (IgV) motif and 3 Ig-like C2-type (IgC2) motifs in the N-terminal extracellular domain (ECD), followed by a transmembrane domain (TM) and an ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 has 3 soluble and 7 transmembrane isoforms due to alternate splicing. The functions of these isoforms are not clear. CEACAM1 is an adhesion molecule mediating cell adhesion via both homotypic and heterotypic associations with CEACAM5/CEA and CEACAM6/NCA.  CEACAM1 is highly glycosylated and can be detected on leukocytes, epithelia, and endothelia. CEACAM1 is involved in multiple cellular processes including differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.

 

Product Details

 

Gene Symbol: CEACAM1; CD66a; BGP; BGP1; BGPI

 

NCBI Gene ID: 634

 

Uniprot Entry: P13688

 

Construct Details: The recombinant human CEACAM1-Fc is expressed as a 632-amino acid protein consisting of Gln35 -  Gly428 region of CEACAM1 (UniProt accession #P13688) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.   

   

Source: Human cells stably expressing human CEACAM1-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QLTTESMPFN VAEGKEVLLL VHNLPQQLFG YSWYKGERVD GNRQIVGYAI GTQQATPGPA 

        70         80         90        100        110        120 

NSGRETIYPN ASLLIQNVTQ NDTGFYTLQV IKSDLVNEEA TGQFHVYPEL PKPSISSNNS 

       130        140        150        160        170        180 

NPVEDKDAVA FTCEPETQDT TYLWWINNQS LPVSPRLQLS NGNRTLTLLS VTRNDTGPYE 

       190        200        210        220        230        240 

CEIQNPVSAN RSDPVTLNVT YGPDTPTISP SDTYYRPGAN LSLSCYAASN PPAQYSWLIN  

       250        260        270        280        290        300 

GTFQQSTQEL FIPNITVNNS GSYTCHANNS VTGCNRTTVK TIIVTELSPV VAKPQIKASK 

       310        320        330        340        350        360 

TTVTGDKDSV NLTCSTNDTG ISIRWFFKNQ SLPSSERMKL SQGNTTLSIN PVKREDAGTY 

       370        380        390        400        410        420 

WCEVFNPISK NQSDPIMLNV NYNALPQENG LSPGSTTENL YFQGSTGTHT CPPCPAPELL 

       430        440        450        460        470        480 

GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ 

       490        500        510        520        530        540 

YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR 

       550        560        570        580        590        600 

EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS 

       610        620        630 

RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK 

 

 

M.W.: Calculated molecular mass (kDa): 70; Estimated by SDS-PAGE under reducing condition (kDa): 110-120

 

Calculated PI: 5.70

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 94490

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Inhibits anti-CD3 antibody-induced IL-2 production in human T cells.

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 85:4648 (1988).

2. Mol. Cell. Biol. 10: 2738 (1990).

3. Cancer Res. 51:5679 (1991).

4. J. Exp. Med. 185:1557 (1997).

5. Semin. Cancer Biol. 9:67 (1999).

6. J. Biol. Chem. 275: 26935 (2000).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2534B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.