The anti-control antigen & anti-CD3e bispecific antibody (scFv-scFv’) recognizes a synthetic antigen that is not found in any mammalian tissues, but binds human T cell antigen CD3e.  The anti-CD3e scFv chain has the same sequence and orientation in this control as in all other bispecific antibodies.  This bispecific antibody does not engage any known antigen and therefore may be used as a control when testing  T-cell-mediated anti-tumor activity by a bispecific antibody.

The anti-control antigen & anti-CD3e bispecific antibody (scFv-scFv’) recognizes a synthetic antigen that is not found in any mammalian tissues, but binds human T cell antigen CD3e.  The anti-CD3e scFv chain has the same sequence and orientation in this control as in all other bispecific antibodies.  This bispecific antibody does not engage any known antigen and therefore may be used as a control when testing  T-cell-mediated anti-tumor activity by a bispecific antibody.

 

Product Details

 

Gene Symbol: CD3E; T3E; TCRE; IMD18; Leu-4

 

NCBI Gene ID: 916 

 

Uniprot Entry: P07766

  

Construct Details: Expressed as a single-chain Fv (scFv) from anti-control antigen VH and VL region fused with a scFv of anti-CD3e VH and VL region with a His-tag at the C-terminus. Exists as a monomer under non-reducing and reducing condition.

 

Source: Mammalian cells stably expressing anti-control:anti-CD3e bispecific antibody (in a scFv-scFv’ format) and growing in chemical-defined media with no animal component or antibiotics.

 

M.W.: Calculated molecular mass (kDa): 55.8. Estimated by SDS-PAGE under non-reducing condition(kDa): 55-60. Calculated extinction coefficients (M-1 cm-1, at 280nm):  115795.

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). The purified recombinant monoclonal antibody was labeled with Biotin (3-5 Biotin per IgG) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Not available.

 

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2576B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.