CSF1R (colony stimulating factor 1 receptor), also known as M-CSFR (macrophage colony-stimulating factor receptor) and CD115, is encoded by the proto-oncogene c-fms.  CSF1R is a 1 pass type I transmembrane protein of the CSF1/PDGF receptor subfamily of receptor tyrosine kinases (RTKs). Like other members, CSF1R contains 5 immunoglobulin­like (Ig) domains in their extracellular region and a tyrosine kinase domain in their intracellular region. CSF1R acts as a receptor for CSF1 and IL34, and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. CSF1R promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration.  CSF1R promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. CSF1R also regulates osteoclast proliferation and differentiation and is required for normal bone and tooth development. CSF1R is also required for normal male and female fertility, and for development of milk ducts and acinar structures in the mammary gland during pregnancy. CSF1R activates several signaling pathways including PKC, AKT MAPK and SRC kinase pathways. CSF1R also activates STAT family members.  Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy. Aberrant expression of CSF1R is observed in breast, ovarian, prostate, and endometrial cancers as well as inflammatory diseases.

CSF1R (colony stimulating factor 1 receptor), also known as M-CSFR (macrophage colony-stimulating factor receptor) and CD115, is encoded by the proto-oncogene c-fms.  CSF1R is a 1 pass type I transmembrane protein of the CSF1/PDGF receptor subfamily of receptor tyrosine kinases (RTKs). Like other members, CSF1R contains 5 immunoglobulin­like (Ig) domains in their extracellular region and a tyrosine kinase domain in their intracellular region. CSF1R acts as a receptor for CSF1 and IL34, and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. CSF1R promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration.  CSF1R promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. CSF1R also regulates osteoclast proliferation and differentiation and is required for normal bone and tooth development. CSF1R is also required for normal male and female fertility, and for development of milk ducts and acinar structures in the mammary gland during pregnancy. CSF1R activates several signaling pathways including PKC, AKT MAPK and SRC kinase pathways. CSF1R also activates STAT family members.  Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy. Aberrant expression of CSF1R is observed in breast, ovarian, prostate, and endometrial cancers as well as inflammatory diseases.

 

Product Details

 

Gene Symbol: CSF1R; FMS; M-CSFR; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R

 

NCBI Gene ID: 1436

 

Uniprot Entry: P07333

 

Construct Details: The recombinant human CSF1R-Fc is expressed as a 736 -amino acid protein consisting of Ile20 - Pro517 region of CSF1R with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.   

  

Source: Human cells stably expressing human CSF1R-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

IPVIEPSVPE LVVKPGATVT LRCVGNGSVE WDGPPSPHWT LYSDGSSSIL STNNATFQNT 

        70         80         90        100        110        120 

GTYRCTEPGD PLGGSAAIHL YVKDPARPWN VLAQEVVVFE DQDALLPCLL TDPVLEAGVS  

       130        140        150        160        170        180 

LVRVRGRPLM RHTNYSFSPW HGFTIHRAKF IQSQDYQCSA LMGGRKVMSI SIRLKVQKVI 

       190        200        210        220        230        240 

PGPPALTLVP AELVRIRGEA AQIVCSASSV DVNFDVFLQH NNTKLAIHQQ SDFHNNRYQK 

       250        260        270        280        290        300 

VLTLNLDQVD FQHAGNYSCV ASNVQGKHST SMFFRVVESA YLNLSSEQNL IQEVTVGEGL  

       310        320        330        340        350        360 

NLKVMVEAYP GLQGFNWTYL GPFSDHQPEP KLANVTTKDT YRHTFTLSLP RLKPSEAGRY 

       370        380        390        400        410        420 

SFLARNPGGW RALTFELTLR YPPEVSVIWT FINGSGTLLC AASGYPQPNV TWLQCSGHTD 

       430        440        450        460        470        480 

RCDEAQVLQV WDDPYPEVLS QEPFHKVTVQ SLLTVETLEH NQTYECRAHN SVGSGSWAFI 

       490        500        510        520        530        540 

PISAGAHTHP PDEFLFTPST TENLYFQGST GTHTCPPCPA PELLGGPSVF LFPPKPKDTL 

       550        560        570        580        590        600 

MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ 

       610        620        630        640        650        660 

DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG 

       670        680        690        700        710        720 

FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA  

       730 

LHNHYTQKSL SLSPGK

 

  

M.W.: Calculated molecular mass (kDa): 81.9 Estimated by SDS-PAGE under reducing condition (kDa): 90-100.

 

Calculated PI: 6.22

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 116740

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds human CSF1 (SKU#FCL2669) and neutralizes CSF1-mediated signaling activity including CSF1-induced proliferation of leukemia lymphoblast cells.

 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Cell 42:421-428(1985)

2. Nature 320:277-280(1986)

3. Oncogene Res. 4:9-17(1989)

4. Cancer Res. 69:9498-9506(2009) 

5. Cytokine 52:215-220(2010)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2648B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.