CD13, also known as ANPEP (alanyl aminopeptidase), APM (aminopeptidase M) and APN (aminopeptidase N), is a single pass type II transmembrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a 935 amino acid (aa) extracellular domain (ECD). The large ECD contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. CD13 is a broad specificity aminopeptidase belonging to the Peptidase M1 family. CD13 may be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines.  CD13 located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. CD13 cleaves antigen peptides bound to major histocompatibility complex class II molecules of antigen presenting cells and to degrade neurotransmitters at synaptic junctions. CD13 Is also implicated as a regulator of IL-8 bioavailability in the endometrium, contributing to angiogenesis.  CD13 is a receptor for human coronavirus that is a cause of upper respiratory tract infections. It also mediates human cytomegalovirus (HCMV) infection. Defects in the CD13 gene may be a cause of various types of leukemia or lymphoma. CD13 plays a critical role in the pathogenesis of cholesterol gallstone disease. 

CD13, also known as ANPEP (alanyl aminopeptidase), APM (aminopeptidase M) and APN (aminopeptidase N), is a single pass type II transmembrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a 935 amino acid (aa) extracellular domain (ECD). The large ECD contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. CD13 is a broad specificity aminopeptidase belonging to the Peptidase M1 family. CD13 may be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines.  CD13 located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. CD13 cleaves antigen peptides bound to major histocompatibility complex class II molecules of antigen presenting cells and to degrade neurotransmitters at synaptic junctions. CD13 Is also implicated as a regulator of IL-8 bioavailability in the endometrium, contributing to angiogenesis.  CD13 is a receptor for human coronavirus that is a cause of upper respiratory tract infections. It also mediates human cytomegalovirus (HCMV) infection. Defects in the CD13 gene may be a cause of various types of leukemia or lymphoma. CD13 plays a critical role in the pathogenesis of cholesterol gallstone disease. 

 

Product Details

 

Gene Symbol: ANPEP; APN; CD13; LAP1; P150; PEPN; GP150

 

NCBI Gene ID: 290

 

Uniprot Entry: P15144

 

Construct Details: The recombinant human CD13-Fc protein is expressed as a 1161 amino acid protein consisting of Gln34 - Lys767 region of CD13 fused to the Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing human CD13-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QEKNKNANSS PVASTTPSAS ATTNPASATT LDQSKAWNRY RLPNTLKPDS YRVTLRPYLT 

        70         80         90        100        110        120 

PNDRGLYVFK GSSTVRFTCK EATDVIIIHS KKLNYTLSQG HRVVLRGVGG SQPPDIDKTE 

       130        140        150        160        170        180 

LVEPTEYLVV HLKGSLVKDS QYEMDSEFEG ELADDLAGFY RSEYMEGNVR KVVATTQMQA  

       190        200        210        220        230        240 

ADARKSFPCF DEPAMKAEFN ITLIHPKDLT ALSNMLPKGP STPLPEDPNW NVTEFHTTPK 

       250        260        270        280        290        300 

MSTYLLAFIV SEFDYVEKQA SNGVLIRIWA RPSAIAAGHG DYALNVTGPI LNFFAGHYDT 

       310        320        330        340        350        360 

PYPLPKSDQI GLPDFNAGAM ENWGLVTYRE NSLLFDPLSS SSSNKERVVT VIAHELAHQW 

       370        380        390        400        410        420 

FGNLVTIEWW NDLWLNEGFA SYVEYLGADY AEPTWNLKDL MVLNDVYRVM AVDALASSHP 

       430        440        450        460        470        480 

LSTPASEINT PAQISELFDA ISYSKGASVL RMLSSFLSED VFKQGLASYL HTFAYQNTIY 

       490        500        510        520        530        540 

LNLWDHLQEA VNNRSIQLPT TVRDIMNRWT LQMGFPVITV DTSTGTLSQE HFLLDPDSNV 

       550        560        570        580        590        600 

TRPSEFNYVW IVPITSIRDG RQQQDYWLID VRAQNDLFST SGNEWVLLNL NVTGYYRVNY 

       610        620        630        640        650        660 

DEENWRKIQT QLQRDHSAIP VINRAQIIND AFNLASAHKV PVTLALNNTL FLIEERQYMP 

       670        680        690        700        710        720 

WEAALSSLSY FKLMFDRSEV YGPMKNYLKK QVTPLFIHFR NNTNNWREIP ENLMDQYSEV 

       730        740        750        760        770        780 

NAISTACSNG VPECEEMVSG LFKQWMENPN NNPIHPNLRS TVYCNAIAQG GEEEWDFAWE 

       790        800        810        820        830        840 

QFRNATLVNE ADKLRAALAC SKELWILNRY LSYTLNPDLI RKQDATSTII SITNNVIGQG 

       850        860        870        880        890        900 

LVWDFVQSNW KKLFNDYGGG SFSFSNLIQA VTRRFSTEYE LQQLEQFKKD NEETGFGSGT 

       910        920        930        940        950        960 

RALEQALEKT KANIKWVKEN KEVVLQWFTE NSKSTGTHTC PPCPAPELLG GPSVFLFPPK 

       970        980        990       1000       1010       1020 

PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL 

      1030       1040       1050       1060       1070       1080 

TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT 

      1090       1100       1110       1120       1130       1140 

CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS 

      1150       1160 

VMHEALHNHY TQKSLSLSPG K 

  

M.W.: Calculated molecular mass (kDa): 131.6; Estimated by SDS-PAGE under reducing condition (kDa): 180-190

 

Calculated PI: 5.51

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 230280

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.