ROR2 (receptor tyrosine kinase-like orphan receptor 2), also known as NTRKR2 (neurotrophic tyrosine kinase, receptor-related 2), is a single-pass type I transmembrane glycoprotein that belongs to the ROR subfamily of the tyrosine protein kinase family. The ROR subfamily members, ROR1 and ROR2, are tyrosine kinase receptors important in regulating skeletal and neuronal development, cell migration and cell polarity. Each ROR member contains 1 FZ (frizzled) domain, 1 Ig (immunoglobulin)-like C2-type domain, and 1 kringle domain in the extracellular region and 1 protein kinase domain in the cytoplasmic region. ROR2 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.  ROR2 may be involved in the early formation of the chondrocytes and required for cartilage and growth plate development. ROR2 exists as a homodimer on the cell surface and may mediate a signaling pathway that promotes osteogenesis and bone formation. Mutations in the ROR2 gene can cause brachydactyly type B, a skeletal disorder characterized by hypoplasia/aplasia of distal phalanges and nails. In addition, mutations in this gene can cause the autosomal recessive form of Robinow syndrome, which is characterized by skeletal dysplasia with generalized limb bone shortening, segmental defects of the spine, brachydactyly, and a dysmorphic facial appearance. ROR2 appears to possess dual roles as a tumor suppressor or activator depending on tumor type.

ROR2 (receptor tyrosine kinase-like orphan receptor 2), also known as NTRKR2 (neurotrophic tyrosine kinase, receptor-related 2), is a single-pass type I transmembrane glycoprotein that belongs to the ROR subfamily of the tyrosine protein kinase family. The ROR subfamily members, ROR1 and ROR2, are tyrosine kinase receptors important in regulating skeletal and neuronal development, cell migration and cell polarity. Each ROR member contains 1 FZ (frizzled) domain, 1 Ig (immunoglobulin)-like C2-type domain, and 1 kringle domain in the extracellular region and 1 protein kinase domain in the cytoplasmic region. ROR2 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.  ROR2 may be involved in the early formation of the chondrocytes and required for cartilage and growth plate development. ROR2 exists as a homodimer on the cell surface and may mediate a signaling pathway that promotes osteogenesis and bone formation. Mutations in the ROR2 gene can cause brachydactyly type B, a skeletal disorder characterized by hypoplasia/aplasia of distal phalanges and nails. In addition, mutations in this gene can cause the autosomal recessive form of Robinow syndrome, which is characterized by skeletal dysplasia with generalized limb bone shortening, segmental defects of the spine, brachydactyly, and a dysmorphic facial appearance. ROR2 appears to possess dual roles as a tumor suppressor or activator depending on tumor type.

 

Product Details

 

Gene Symbol: BDB; BDB1; NTRKR2

 

NCBI Gene ID: 4920

 

Uniprot Entry: Q01974

 

Construct Details: The recombinant human ROR2-Fc fusion is expressed as a 598 amino acid protein consisting of Glu34 - Gly403 region of ROR2 (Uniprot #Q01974) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).

 

Source:  Human cells stably expressing ROR2-Fc and growing in chemical-defined media with no animal components or antibiotics.

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

EVEVLDPNDP LGPLDGQDGP IPTLKGYFLN FLEPVNNITI VQGQTAILHC KVAGNPPPNV 

        70         80         90        100        110        120 

RWLKNDAPVV QEPRRIIIRK TEYGSRLRIQ DLDTTDTGYY QCVATNGMKT ITATGVLFVR 

       130        140        150        160        170        180 

LGPTHSPNHN FQDDYHEDGF CQPYRGIACA RFIGNRTIYV DSLQMQGEIE NRITAAFTMI 

       190        200        210        220        230        240 

GTSTHLSDQC SQFAIPSFCH FVFPLCDARS RTPKPRELCR DECEVLESDL CRQEYTIARS 

       250        260        270        280        290        300 

NPLILMRLQL PKCEALPMPE SPDAANCMRI GIPAERLGRY HQCYNGSGMD YRGTASTTKS  

       310        320        330        340        350        360 

GHQCQPWALQ HPHSHHLSST DFPELGGGHA YCRNPGGQME GPWCFTQNKN VRMELCDVPS 

       370        380        390        400        410        420 

CSPRDSSKMG STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE 

       430        440        450        460        470        480 

DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP  

       490        500        510        520        530        540 

APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN  

       550        560        570        580        590 

NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK

 

M.W.: Calculated molecular mass (kDa): 66.9; Estimated by SDS-PAGE under reducing condition (kDa): 75-85

 

Calculated PI: 6.49

 

Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 71290

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Not Available

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Biol. Chem. 267:26181 (1992). 

2. Nat. Genet. 24:275 (2000).

3. Am. J. Hum. Genet. 67:822 (2000).

4. Nat. Genet. 25:419 (2000).

5. Nat. Genet. 25:423 (2000).

6. Mol. Endocrinol. 21:3050 (2007).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0229B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.