B7-H7 (B7 homolog 7), also known as HHLA2 (human endogenous retrovirus-H long terminal repeat-associating protein 2), is regarded as a new member of the B7 ligand family. B7-H7 contains 1 Ig-like (immunoglobulin-like) C1-type and 2 Ig-like V-type domains while other members are typically composed of 1 Ig-like V-type and 1 Ig-like C1-type domains.  Interactions between members of the B7 ligand and CD28/CTLA4 receptor families can generate positive co-stimulation and negative co-inhibition in regulating T cell responses. B7-H7 is expressed at high levels in colon, kidney, testis, lung and pancreas, and at lower levels in small intestine, liver and skeletal muscle. In immune cells, B7-H7 is highly expressed in B-cells, dendritic cells, monocytes and macrophages, but not detected in T-cells. B7-H7 was reported to regulate cell-mediated immunity by binding to a putative receptor on T lymphocytes and inhibiting the proliferation of T cells. B7-H7 has also been referred to as B-H5 (B7 homolog 5), which was identified as a specific ligand for CD28H (CD28 homolog) or TMIGD2 (transmembrane and Ig domain-containing protein 2). B7-H5 is constitutively found in macrophages and could be induced on dendritic cells in response to inflammation. The B7-H5/CD28H interaction was shown to selectively co-stimulate human T-cell growth and cytokine production via an AKT-dependent signalling cascade.

B7-H7 (B7 homolog 7), also known as HHLA2 (human endogenous retrovirus-H long terminal repeat-associating protein 2), is regarded as a new member of the B7 ligand family. B7-H7 contains 1 Ig-like (immunoglobulin-like) C1-type and 2 Ig-like V-type domains while other members are typically composed of 1 Ig-like V-type and 1 Ig-like C1-type domains.  Interactions between members of the B7 ligand and CD28/CTLA4 receptor families can generate positive co-stimulation and negative co-inhibition in regulating T cell responses. B7-H7 is expressed at high levels in colon, kidney, testis, lung and pancreas, and at lower levels in small intestine, liver and skeletal muscle. In immune cells, B7-H7 is highly expressed in B-cells, dendritic cells, monocytes and macrophages, but not detected in T-cells. B7-H7 was reported to regulate cell-mediated immunity by binding to a putative receptor on T lymphocytes and inhibiting the proliferation of T cells. B7-H7 has also been referred to as B-H5 (B7 homolog 5), which was identified as a specific ligand for CD28H (CD28 homolog) or TMIGD2 (transmembrane and Ig domain-containing protein 2). B7-H5 is constitutively found in macrophages and could be induced on dendritic cells in response to inflammation. The B7-H5/CD28H interaction was shown to selectively co-stimulate human T-cell growth and cytokine production via an AKT-dependent signalling cascade.

 

Product Details

 

Gene Symbol: B7-H7; HHLA2; B7H7; B7-H5; B7H5

 

NCBI Gene ID: 11148

 

Uniprot Entry: Q9UM44

 

Construct Details: The recombinant human B7-H7-Fc fusion protein is expressed as a 574 amino acid protein consisting of Ile23 - Lys345 region of HHLA2/B7-H7 (UniProt accession #Q9UM44) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.

 

Source:  Human cells stably expressing B7-H7-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

MKAQTALSFFLILITSLSGSQGIFPLAFFIYVPMNEQIVIGRLDEDIILPSSFERGSEVVIHWKYQDSYK

VHSYYKGSDHLESQDPRYANRTSLFYNEIQNGNASLFFRRVSLLDEGIYTCYVGTAIQVITNKVVLKVGV

FLTPVMKYEKRNTNSFLICSVLSVYPRPIITWKMDNTPISENNMEETGSLDSFSINSPLNITGSNSSYEC

TIENSLLKQTWTGRWTMKDGLHKMQSEHVSLSCQPVNDYFSPNQDFKVTWSRMKSGTFSVLAYYLSSSQN

TIINESRFSWNKELINQSDFSMNLMDLNLSDSGEYLCNISSDEYTLLTIHTVHVEPSQETASHNKGSTGT

HTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPRE

EQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQV

SLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALH

NHYTQKSLSLSPGK

 

M.W.:  Calculated molecular mass 65.0 kDa; estimated by SDS-PAGE under reducing condition ~75 kDa probably due to glycosylation

 

Calculated PI: 6.04

 

Calculated Extinction Coefficients (M-1 cm-1, at 280nm):  94365

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Inhibits anti-CD3 antibody induced cytokine (e.g., IFN- and IL-2) secretion in human T lymphocytes

  

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Genomics 59:255-63 (1999).

2. Immunogenetics. 64:571-90 (2012).

3. Nat. Commun. 4:2043-2043 (2013)

4. Proc. Natl. Acad. Sci. 110:9879-84 (2013).

 

 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL1038-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.