CD130, also known as IL6ST (interleukin 6 signal transducer), IL6RB (Interleukin-6 receptor subunit beta) and gp130, is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 2 subfamily.  CD130/IL6ST consists of an extracellular region with 1 Ig (immunoglobulin)-like C2-type dpomain, 5 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation.  IL6ST/CD130 is a common signal-transducing molecule shared by many cytokines including IL6, LIF, OSM, CNTF, IL11 and CT1 which utilize IL6ST for initiating signal transmission. For example, binding of IL6 to IL6R/CD126 induces IL6ST homodimerization and formation of a high-affinity receptor complex. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass IL6R/CD126 and directly activate IL6ST. IL6ST mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism.  It plays a role in embryonic development and is essential for survival of motor and sensory neurons and for differentiation of astrocytes.  IL6ST is required for normal trabecular bone mass and cortical bone composition.

CD130, also known as IL6ST (interleukin 6 signal transducer), IL6RB (Interleukin-6 receptor subunit beta) and gp130, is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 2 subfamily.  CD130/IL6ST consists of an extracellular region with 1 Ig (immunoglobulin)-like C2-type dpomain, 5 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with a box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction and activation.  IL6ST/CD130 is a common signal-transducing molecule shared by many cytokines including IL6, LIF, OSM, CNTF, IL11 and CT1 which utilize IL6ST for initiating signal transmission. For example, binding of IL6 to IL6R/CD126 induces IL6ST homodimerization and formation of a high-affinity receptor complex. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass IL6R/CD126 and directly activate IL6ST. IL6ST mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism.  It plays a role in embryonic development and is essential for survival of motor and sensory neurons and for differentiation of astrocytes.  IL6ST is required for normal trabecular bone mass and cortical bone composition.

 

Product Details

 

Gene Symbol: IL6ST; CD130; GP130; IL6RB; CDw130; IL-6RB

 

NCBI Gene ID: 3572

 

Uniprot Entry: P40189

 

Construct Details: The recombinant human CD130-Fc protein is expressed as a 825 amino acid protein consisting of Glu23 - Glu619 region of CD130 fused to the Fc from human IgG1, which exists as a dimer under non-reducing conditions.  CD130 ECD contains 10 potential N-linked glycosylation sites.

 

Source: Human cells stably expressing CD130-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

ELLDPCGYIS PESPVVQLHS NFTAVCVLKE KCMDYFHVNA NYIVWKTNHF TIPKEQYTII 

        70         80         90        100        110        120 

NRTASSVTFT DIASLNIQLT CNILTFGQLE QNVYGITIIS GLPPEKPKNL SCIVNEGKKM 

       130        140        150        160        170        180 

RCEWDGGRET HLETNFTLKS EWATHKFADC KAKRDTPTSC TVDYSTVYFV NIEVWVEAEN 

       190        200        210        220        230        240 

ALGKVTSDHI NFDPVYKVKP NPPHNLSVIN SEELSSILKL TWTNPSIKSV IILKYNIQYR 

       250        260        270        280        290        300 

TKDASTWSQI PPEDTASTRS SFTVQDLKPF TEYVFRIRCM KEDGKGYWSD WSEEASGITY 

       310        320        330        340        350        360 

EDRPSKAPSF WYKIDPSHTQ GYRTVQLVWK TLPPFEANGK ILDYEVTLTR WKSHLQNYTV 

       370        380        390        400        410        420 

NATKLTVNLT NDRYLATLTV RNLVGKSDAA VLTIPACDFQ ATHPVMDLKA FPKDNMLWVE 

       430        440        450        460        470        480 

WTTPRESVKK YILEWCVLSD KAPCITDWQQ EDGTVHRTYL RGNLAESKCY LITVTPVYAD 

       490        500        510        520        530        540 

GPGSPESIKA YLKQAPPSKG PTVRTKKVGK NEAVLEWDQL PVDVQNGFIR NYTIFYRTII 

       550        560        570        580        590        600 

GNETAVNVDS SHTEYTLSSL TSDTLYMVRM AAYTDEGGKD GPEFTFTTPK FAQGEIEVDK 

       610        620        630        640        650        660 

THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV 

       670        680        690        700        710        720 

EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ 

       730        740        750        760        770        780 

PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG 

       790        800        810        820 

SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK 

 

 

  

M.W.: Calculated molecular mass (kDa): 93.5; Estimated by SDS-PAGE under reducing condition (kDa): ~100

 

Calculated PI: 6.30

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  165130

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.