LILRB1 (Leukocyte immunoglobulin like receptor B1), also known as CD85J, LIR1 (Leukocyte immunoglobulin-like receptor 1), ILT2 (Ig-like transcript 2), and MIR7 (myeloid inhibitory receptor 7), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF).  It belongs to the subfamily B class of LIR receptors (LILRBs), which are single-pass type I trnasmembrane proteins with 2 or 4 extracellular Ig-like domains, a transmembrane domain (TM), and 2-4 cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). LILRB1 contains 4 Ig-like C2 type domains and 4 TIIMs. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases to modulate cellular responses.  LILRB1 is expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. It is also detected on a low percentage of T-cells and natural killer (NK) cells.  LILRB1 binds to MHC class I molecules on antigen-presenting cells (APCs) and transduces a negative signal that inhibits stimulation of an immune response. LILRB1 may control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. LILRB1 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. LILRB1 also binds FCER1A and FCGR1A. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release.  LILRB1 acts as a receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. 

LILRB1 (Leukocyte immunoglobulin like receptor B1), also known as CD85J, LIR1 (Leukocyte immunoglobulin-like receptor 1), ILT2 (Ig-like transcript 2), and MIR7 (myeloid inhibitory receptor 7), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF).  It belongs to the subfamily B class of LIR receptors (LILRBs), which are single-pass type I trnasmembrane proteins with 2 or 4 extracellular Ig-like domains, a transmembrane domain (TM), and 2-4 cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). LILRB1 contains 4 Ig-like C2 type domains and 4 TIIMs. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases to modulate cellular responses.  LILRB1 is expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. It is also detected on a low percentage of T-cells and natural killer (NK) cells.  LILRB1 binds to MHC class I molecules on antigen-presenting cells (APCs) and transduces a negative signal that inhibits stimulation of an immune response. LILRB1 may control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. LILRB1 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. LILRB1 also binds FCER1A and FCGR1A. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release.  LILRB1 acts as a receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. 

 

Product Details

 

Gene Symbol: LILRB1; ILT2; LIR1; MIR7; PIRB; CD85J; ILT-2; LIR-1; MIR-7; PIR-B

 

NCBI Gene ID: 10859

 

Uniprot Entry: Q8NHL6

 

Construct Details: The recombinant human LILRB1-Fc fusion is expressed as a 665 amino acid protein consisting of Gly24 - Val461 region of LILRB1 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing human LILRB1-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

GHLPKPTLWA EPGSVITQGS PVTLRCQGGQ ETQEYRLYRE KKTAPWITRI PQELVKKGQF 

        70         80         90        100        110        120 

PIPSITWEHT GRYRCYYGSD TAGRSESSDP LELVVTGAYI KPTLSAQPSP VVNSGGNVTL  

       130        140        150        160        170        180 

QCDSQVAFDG FILCKEGEDE HPQCLNSQPH ARGSSRAIFS VGPVSPSRRW WYRCYAYDSN 

       190        200        210        220        230        240 

SPYEWSLPSD LLELLVLGVS KKPSLSVQPG PIVAPEETLT LQCGSDAGYN RFVLYKDGER  

       250        260        270        280        290        300 

DFLQLAGAQP QAGLSQANFT LGPVSRSYGG QYRCYGAHNL SSEWSAPSDP LDILIAGQFY 

       310        320        330        340        350        360 

DRVSLSVQPG PTVASGENVT LLCQSQGWMQ TFLLTKEGAA DDPWRLRSTY QSQKYQAEFP 

       370        380        390        400        410        420 

MGPVTSAHAG TYRCYGSQSS KPYLLTHPSD PLELVVSGPS GGPSSPTTGP TSTSGPEDQP 

       430        440        450        460        470        480 

LTPTGSDPQS GLGRHLGVDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV 

       490        500        510        520        530        540 

VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK 

       550        560        570        580        590        600 

VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE 

       610        620        630        640        650        660 

SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS 

  

LSPGK 

  

M.W.: Calculated molecular mass (kDa): 73.1; Estimated by SDS-PAGE under reducing condition (kDa): 2 major bands 75-85 & 50-60 kDa probably due to proteolytic cleavage within the ECD of LILRB1

 

Calculated PI: 6.24

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 90730

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. J. Exp. Med. 186:1809-1818(1997)

2. J. Immunol. 159:5192-5196(1997)

3. Eur. J. Immunol. 28:3423-3434(1998)

4. Immunity 11:603-613(1999) 

5. J. Immunol. 168:3351-3359(2002)

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.