MSR1 (macrophage scavenger receptor 1), also known as CD204 and SCARA1 (scavenger receptor class A member 1), is a single-pass, type II membrane protein.  It belongs to an expanding family of transmembrane molecules collectively referred to as scavenger receptors.  Receptors of this family bind to a series of negatively­charged/polyanionic ligands.  They recognize and internalize modified low density lipoproteins (LDLs) in the removal of many foreign substances and waste materials in the living body. Scavenger receptors can be categorized into 3 classes, and MSR1 is the class A receptors with 3 different types generated by alternative splicing. The extracellular region of MSR1 contains an α­helical coiled­coil domain, a collagen­like domain, and a cysteine­rich domain (CRD) at the C-terminus. MSR1 is a macrophage-specific trimeric transmembrane glycoprotein involved in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 (MSR-AI or SR-AI) and type 2 (MSR-AII or SR-AII) are functional receptors, which mediate the endocytosis of modified LDLs. The isoform type 3 (MSR-AIII or SR-AIII) does not internalize modified LDLs. It has an altered intracellular processing and is trapped within the endoplasmic reticulum.  Thus the isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect. MSR1 may also play important roles in macrophage growth, cell adhesion, and osteoclast differentiation.

MSR1 (macrophage scavenger receptor 1), also known as CD204 and SCARA1 (scavenger receptor class A member 1), is a single-pass, type II membrane protein.  It belongs to an expanding family of transmembrane molecules collectively referred to as scavenger receptors.  Receptors of this family bind to a series of negatively­charged/polyanionic ligands.  They recognize and internalize modified low density lipoproteins (LDLs) in the removal of many foreign substances and waste materials in the living body. Scavenger receptors can be categorized into 3 classes, and MSR1 is the class A receptors with 3 different types generated by alternative splicing. The extracellular region of MSR1 contains an α­helical coiled­coil domain, a collagen­like domain, and a cysteine­rich domain (CRD) at the C-terminus. MSR1 is a macrophage-specific trimeric transmembrane glycoprotein involved in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 (MSR-AI or SR-AI) and type 2 (MSR-AII or SR-AII) are functional receptors, which mediate the endocytosis of modified LDLs. The isoform type 3 (MSR-AIII or SR-AIII) does not internalize modified LDLs. It has an altered intracellular processing and is trapped within the endoplasmic reticulum.  Thus the isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect. MSR1 may also play important roles in macrophage growth, cell adhesion, and osteoclast differentiation.

 

Product Details

 

Gene Symbol: MSR1; SRA; SR-A; CD204; phSR1; phSR2; SCARA1

 

NCBI Gene ID: 4481

 

Uniprot Entry: P21757

 

Construct Details: The recombinant human MSR1/CD204-Fc fusion is expressed as a 603 amino acid protein consisting of Lys77 - Leu451 region of MSR1/CD204 (Uniprot Accession #P21757) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing MSR1-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

KWETKNCSVS STNANDITQS LTGKGNDSEE EMRFQEVFME HMSNMEKRIQ HILDMEANLM 

        70         80         90        100        110        120 

DTEHFQNFSM TTDQRFNDIL LQLSTLFSSV QGHGNAIDEI SKSLISLNTT LLDLQLNIEN 

       130        140        150        160        170        180 

LNGKIQENTF KQQEEISKLE ERVYNVSAEI MAMKEEQVHL EQEIKGEVKV LNNITNDLRL 

       190        200        210        220        230        240 

KDWEHSQTLR NITLIQGPPG PPGEKGDRGP TGESGPRGFP GPIGPPGLKG DRGAIGFPGS 

       250        260        270        280        290        300 

RGLPGYAGRP GNSGPKGQKG EKGSGNTLTP FTKVRLVGGS GPHEGRVEIL HSGQWGTICD 

       310        320        330        340        350        360 

DRWEVRVGQV VCRSLGYPGV QAVHKAAHFG QGTGPIWLNE VFCFGRESSI EECKIRQWGT 

       370        380        390        400        410        420 

RACSHSEDAG VTCTLSTGTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV 

       430        440        450        460        470        480 

DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS 

       490        500        510        520        530        540 

NKALPAPIEK TISKAKGQPR EPQVYTLPPS REEMTKNQVS LTCLVKGFYP SDIAVEWESN 

       550        560        570        580        590        600 

GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS 

 

PGK 

 

M.W.: Calculated molecular mass (kDa): 66.9; Estimated by SDS-PAGE under reducing condition (kDa): 95-100

 

Calculated PI: 6.03

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 73630

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.25 mg/ml in sterile PBS pH7.4 (carrier and preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to advanced glycation endproducts of bovine serum albumin (AGE­BSA) by ELISA

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 87: 9133 (1990).

2. J. Biol. Chem. 268:2120(1993).

3. J. Lipid Res. 34:983 (1993). 

4. J. Clin. Invest. 108:649 (2001). 

5. Cancer. Res. 63: 3486 (2003).

6. J. Immunol. 173: 6427 (2004).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2292-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.