CLEC10A (C-type lectin domain containing 10A) or CD301, is a single-pass type II transmembrane protein that belongs to a the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily.  Members of this superfamily share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. CLEC10A contains 1 C-type lectin domain in the extracellular region and may play a role in regulating adaptive and innate immune responses. CLEC10A binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells.

CLEC10A (C-type lectin domain containing 10A) or CD301, is a single-pass type II transmembrane protein that belongs to a the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily.  Members of this superfamily share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. CLEC10A contains 1 C-type lectin domain in the extracellular region and may play a role in regulating adaptive and innate immune responses. CLEC10A binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells.

 

Product Details

 

Gene Symbol: CLEC10A; HML; MGL; HML2; CD301; CLECSF13; CLECSF14

 

NCBI Gene ID: 10462

 

Uniprot Entry: Q8IUN9

 

Construct Details: The recombinant human CLEC10A-Fc is expressed as a 484-amino acid protein consisting of Gln61 -  His316 region of CLEC10A and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.  

   

Source: Human cells stably expressing CLEC10A-Fc protein and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
QNSKFQRDLV TLRTDFSNFT SNTVAEIQAL TSQGSSLEET IASLKAEVEG FKQERQAGVS

        70         80         90        100        110        120
ELQEHTTQKA HLGHCPHCPS VCVPVHSEML LRVQQLVQDL KKLTCQVATL NNNASTEGTC

       130        140        150        160        170        180
CPVNWVEHQD SCYWFSHSGM SWAEAEKYCQ LKNAHLVVIN SREEQNFVQK YLGSAYTWMG

       190        200        210        220        230        240
LSDPEGAWKW VDGTDYATGF QNWKPGQPDD WQGHGLGGGE DCAHFHPDGR WNDDVCQRPY

       250        260        270        280        290        300
HWVCEAGLGQ TSQESHSTGT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV

       310        320        330        340        350        360
VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV

       370        380        390        400        410        420
SNKALPAPIE KTISKAKGQP REPQVYTLPP SREEMTKNQV SLTCLVKGFY PSDIAVEWES

       430        440        450        460        470        480
NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL


SPGK

 

 

M.W.: Calculated molecular mass 54.3 kDa; estimated by SDS-PAGE under reducing condition 55-60 kDa

 

Calculated PI: 5.79

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 100350

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining).  The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.