Human CLEC4C/CD303/DLEC/BDCA2 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

CLEC4C (C-type lectin domain family 4 member C) or CD303, is a single-pass type II transmembrane protein that belongs to a the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily.  Members of this superfamily share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. CLEC4C contains 1 C-type lectin domain in the extracellular region and may play a role indendritic cell function.  It is expressed in immature monocyte-derived dendritic cells (iMDDC).  Its expression is significantly down-regulated upon maturation with LPS but not with TNF-alpha. CLEC4C may be involved in antigen-capturing by targeting ligand into antigen processing and peptide-loading compartments for presentation to T-cells. CLEC4C may mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells. It may also act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium.

Gene Symbol: CLEC4C; DLEC; HECL; BDCA2; CD303; CLECSF7; CLECSF11; PRO34150

NCBI Gene ID: 170482

Uniprot Entry: Q8WTT0

Construct Details: The recombinant human CLEC4C-Fc is expressed as a 395-amino acid protein consisting of Met47 -  Ile213 region of CLEC4C and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.  

Source: Human cells stably expressing CLEC4C-Fc protein and growing in chemical-defined media with no animal component or antibiotics

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
MYSKTVKRLS KLREYQQYHP SLTCVMEGKD IEDWSCCPTP WTSFQSSCYF ISTGMQSWTK

        70         80         90        100        110        120
SQKNCSVMGA DLVVINTREE QDFIIQNLKR NSSYFLGLSD PGGRRHWQWV DQTPYNENVT

       130        140        150        160        170        180
FWHSGEPNNL DERCAIINFR SSEEWGWNDI HCHVPQKSIC KMKKIYISTG THTCPPCPAP

       190        200        210        220        230        240
ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR

       250        260        270        280        290        300
EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP

       310        320        330        340        350        360
PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV

       370        380        390
DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK

M.W.: Calculated molecular mass 45.3 kDa; estimated by SDS-PAGE under reducing condition ~55 kDa

Calculated PI: 7.52

Calculated extinction coefficients (M-1 cm-1, at 280nm): 90715

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.