DR4 (death receptor 4), also known as CD261, TRAILR1 and APO-2, is a single pass type 1 transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF10A). DR4 contains an extracellular region with 3 cysteine-rich repeats and a cytoplasmic death domain (DD). DR4 is widely expressed with high levels in spleen, peripheral blood leukocytes, small intestine and thymus. DR4 is detected mainly in damaged, infected and malignant cells.  DR4 is also expressed on hemopoietic cells including activated T-cells and influences activities such as monocyte migration. DR4 is one of 5 receptors for TRAIL (tumor necrosis factor-related apoptosis inducing ligand), also known as Apo2L (Apo-2 ligand) and TNFSF10.  TRAIL preferentially induces apoptosis in tumor cell lines, but not in normal cells. TRAIL binds at least 5 different receptors with characteristic TNFR cysteine-rich repeats. DR4/TRAILR1 is most closely related to DR5/TRAILR2 sharing 59% amino acid identity within the cytoplasmic DD. DR4 transduces cell death signal and induces apoptosis by recruiting a cytosolic death­inducing signaling complex (DISC) that includes the adaptor FADD and caspase 8 and caspase 10. DR4 is thought to function in immune surveillance, inducing apoptosis in cancer cells but not normal cells. DR4 surface expression correlates more closely than that of DR5 with TRAIL induction of apoptosis in tumor cells.

DR4 (death receptor 4), also known as CD261, TRAILR1 and APO-2, is a single pass type 1 transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF10A). DR4 contains an extracellular region with 3 cysteine-rich repeats and a cytoplasmic death domain (DD). DR4 is widely expressed with high levels in spleen, peripheral blood leukocytes, small intestine and thymus. DR4 is detected mainly in damaged, infected and malignant cells.  DR4 is also expressed on hemopoietic cells including activated T-cells and influences activities such as monocyte migration. DR4 is one of 5 receptors for TRAIL (tumor necrosis factor-related apoptosis inducing ligand), also known as Apo2L (Apo-2 ligand) and TNFSF10.  TRAIL preferentially induces apoptosis in tumor cell lines, but not in normal cells. TRAIL binds at least 5 different receptors with characteristic TNFR cysteine-rich repeats. DR4/TRAILR1 is most closely related to DR5/TRAILR2 sharing 59% amino acid identity within the cytoplasmic DD. DR4 transduces cell death signal and induces apoptosis by recruiting a cytosolic death­inducing signaling complex (DISC) that includes the adaptor FADD and caspase 8 and caspase 10. DR4 is thought to function in immune surveillance, inducing apoptosis in cancer cells but not normal cells. DR4 surface expression correlates more closely than that of DR5 with TRAIL induction of apoptosis in tumor cells.

  

Product Details

 

Gene Symbol: TNFRSF10A; APO2; CD261; TRAILR1; TRAIL-R1; TRAILR-1

  

NCBI Gene ID: 8797

 

Uniprot Entry: O00220

 

Construct Details: Full length human DR4 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGGCGCCACCACCAGCTAGAGTACATCTAGGTGCGTTCCTGGCAGTGACTCCGAATCCCGGGAGCGCAGCGAGTGGGAC

AGAGGCAGCCGCGGCCACACCCAGCAAAGTGTGGGGCTCTTCCGCGGGGAGGATTGAACCACGAGGCGGGGGCCGAGGAG

CGCTCCCTACCTCCATGGGACAGCACGGACCCAGTGCCCGGGCCCGGGCAGGGCGCGCCCCAGGACCCAGGCCGGCGCGG

GAAGCCAGCCCTCGGCTCCGGGTCCACAAGACCTTCAAGTTTGTCGTCGTCGGGGTCCTGCTGCAGGTCGTACCTAGCTC

AGCTGCAACCATCAAACTTCATGATCAATCAATTGGCACACAGCAATGGGAACATAGCCCTTTGGGAGAGTTGTGTCCAC

CAGGATCTCATAGATCAGAACATCCTGGAGCCTGTAACCGGTGCACAGAGGGTGTGGGTTACACCAATGCTTCCAACAAT

TTGTTTGCTTGCCTCCCATGTACAGCTTGTAAATCAGATGAAGAAGAGAGAAGTCCCTGCACCACGACCAGGAACACAGC

ATGTCAGTGCAAACCAGGAACTTTCCGGAATGACAATTCTGCTGAGATGTGCCGGAAGTGCAGCAGAGGGTGCCCCAGAG

GGATGGTCAAGGTCAAGGATTGTACGCCCTGGAGTGACATCGAGTGTGTCCACAAAGAATCAGGCAATGGACATAATATA

TGGGTGATTTTGGTTGTGACTTTGGTTGTTCCGTTGCTGTTGGTGGCTGTGCTGATTGTCTGTTGTTGCATCGGCTCAGG

TTGTGGAGGGGACCCCAAGTGCATGGACAGGGTGTGTTTCTGGCGCTTGGGTCTCCTACGAGGGCCTGGGGCTGAGGACA

ATGCTCACAACGAGATTCTGAGCAACGCAGACTCGCTGTCCACTTTCGTCTCTGAGCAGCAAATGGAAAGCCAGGAGCCG

GCAGATTTGACAGGTGTCACTGTACAGTCCCCAGGGGAGGCACAGTGTCTGCTGGGACCGGCAGAAGCTGAAGGGTCTCA

GAGGAGGAGGCTGCTGGTTCCAGCAAATGGTGCTGACCCCACTGAGACTCTGATGCTGTTCTTTGACAAGTTTGCAAACA

TCGTGCCCTTTGACTCCTGGGACCAGCTCATGAGGCAGCTGGACCTCACGAAAAATGAGATCGATGTGGTCAGAGCTGGT

ACAGCAGGCCCAGGGGATGCCTTGTATGCAATGCTGATGAAATGGGTCAACAAAACTGGACGGAACGCCTCGATCCACAC

CCTGCTGGATGCCTTGGAGAGGATGGAAGAGAGACATGCAAGAGAGAAGATTCAGGACCTCTTGGTGGACTCTGGAAAGT

TCATCTACTTAGAAGATGGCACAGGCTCTGCCGTGTCCTTGGAGTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. Science 276:111 (1997).

2. J. Immunol. 175:1232 (2005).

3. Mol. Cancer Ther. 6:2591 (2007).

4. Clin. Cancer Res. 15:5584 (2009).

5. Oncogene 29:4848 (2010).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTPSF54-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.